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| Differences between Gel-filtration and Ion-exchange |
|
Characteristics
|
Gel-filtration
|
Ion-exchange
|
|
1.
Basis of separation
2.
Stationary phase
3.
Mobile phase
4.
Mechanism
5.
Amount off sample applied
6.
Expensive
7.
Effect of pH
8.
Time needed
|
size
of molecules
Porous
gel with no attraction on solute molecules.
Liquid
Smaller
molecules enter the pores and larger molecules pass through column.
Small
such as 3ml
Highly
expensive
No
Time
lengthy.
|
charge
of molecules
Solid
resins carrying fixed ions
Liquid
containing electrolytes
Solute
ions of charge opposite to the fixed ions are attached to resin by
electrostatic forces.
large
Less
expensive
Yes.
pH may be altered.
Not
so much.
|
The Daily Youth- tdy24.com Presents
"Differences
between Gel-filtration and Ion-exchange"
Written By
Sadia Akhtar
Student of Department of Microbiology
Jagannath University.
Email- sadiabd810@yahoo.com
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