Differences between Gel-filtration and Ion-exchange

Differences between Gel-filtration and Ion-exchange
  
Characteristics
Gel-filtration
Ion-exchange
   1.   Basis of separation

   2.   Stationary phase



   3.   Mobile phase


   4.   Mechanism





   5.   Amount off sample applied

   6.   Expensive

   7.   Effect of pH


   8.   Time needed

size of molecules


Porous gel with no attraction on solute molecules.

 Liquid


Smaller molecules enter the pores and larger molecules pass through column.

Small such as 3ml


Highly expensive

No


Time lengthy.
charge of molecules


Solid resins carrying fixed ions


Liquid containing electrolytes

Solute ions of charge opposite to the fixed ions are attached to resin by electrostatic forces.

large


Less expensive

Yes. pmay be altered.

Not so much.


The Daily Youth- tdy24.com Presents
"Differences between Gel-filtration and Ion-exchange"
Written By
Sadia Akhtar
Student of Department of Microbiology
Jagannath University.
Email- sadiabd810@yahoo.com

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